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Crosstalk between MAPK/ERK and PI3K/AKT signal pathways during brain ischemia/reperfusion

Meanwhile, organoids were collected according to the above protocol, except that after the initial centrifugation step, the organoids were resuspended in 3 mL TrypLE Express containing 10 M Y-27632 and incubated in a 37 C water bath for 10 min, followed by trituration 10 times with a P1000 pipette followed by a P10 tip attached to the P1000 tip

Type 1 diabetes occurs when the immune system attacks and destroys beta cells, while Type 2 diabetes involves insulin resistance and eventual beta cell dysfunction

[Abstract]2025 Mar 19;44(1):99